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Sunday, July 12, 2020 | History

2 edition of Structural and functional studies of the Chinese hamster P-glycoprotein gene family. found in the catalog.

Structural and functional studies of the Chinese hamster P-glycoprotein gene family.

Jane Anne.* Endicott

Structural and functional studies of the Chinese hamster P-glycoprotein gene family.

by Jane Anne.* Endicott

  • 79 Want to read
  • 6 Currently reading

Published .
Written in English


The Physical Object
Pagination231 leaves
Number of Pages231
ID Numbers
Open LibraryOL20990657M

Find + million publication pages, 20+ million researchers, and k+ projects. onAcademic is where you discover scientific knowledge and share your research.   Structural studies have been carried out using CHO Lec both in the absence and presence of NB-DNJ which has a complementary inhibition effect to the mutant cell line giving endoglycosidase sensitive sugars (For examples see [46, ]).

The gene was designated MDR3 since it appears to correspond to the third of the P-glycoprotein genes mapped in the hamster. Transcription studies indicated that the MDR3 transcript is nucleotides shorter than the MDR1 transcript as a result of a shorter 3-prime untranslated region of the MDR3 mRNA.   Peptide pheromones regulate developmental processes, including virulence, in Gram-positive bacteria. Immature propeptide pheromones are synthesized, secreted, and undergo proteolytic maturation to serve as intercellular signals. The regulator gene of glucosyltransferase (Rgg) transcription factors are a large family of receptors that directly bind pheromones transported to the cytosol.

Ever since Juliano and Ling first described P-glycoprotein (P-gp) in , 1 it has become an important focus of research. P-gp is a member of the ATP-binding cassette (ABC) superfamily of proteins that is highly conserved in distantly related species (from simple eukaryotes to vertebrates). 2 These similarities across species suggest that P-gp plays an important role in physiological. The lmrA gene specifies a protein that shares structural homology with the well-characterized human P-glycoprotein. Unlike P-glycoprotein, LmrA is a half-transporter which functions as a homodimer. lmrA was expressed in a drug-hypersensitive strain of E. coli CS (tolC) for functional studies.


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Structural and functional studies of the Chinese hamster P-glycoprotein gene family by Jane Anne.* Endicott Download PDF EPUB FB2

Several studies have demonstrated that regulation of P-glycoprotein gene expression at the transcriptional level is complex and involves multiple regulatory mechanisms. To investigate the transcriptional regulation of P-glycoprotein genes, genomic DNA fragments containing the 5' end of the hamster pgp1 and pgp2 genes were isolated and Cited by: 6 Expression of P-glycoprotein Isoforms I.

Introduction II. P-glycoprotein Gene Family Members in Different Species III. Localization of P-glycoprotein Isoforms in Chinese Hamster IV.

Concluding Remarks References 7 The Use of Yeast and Yeast Strains Expressing Human DNA Topoisomerases in the Study of Anticancer Drugs I. Introduction II.

Initially, it was discovered in that P-gp can act as a drug resistance gene while working on the chinese hamster ovary cells. Further studies demonstrated that P-gp intervened resistance used to occur both in-vitro and in-vivo (cultured tumor cell lines and human cancers) (Doyle and Ross, ).Author: Nitika Garg, Rupa Joshi, Bikash Medhi.

Ph.D. in Medical Biophysics, University of Toronto, Canada. “Structural and functional studies of the Chinese hamster P- glycoprotein gene family” Supervisor, Professor Victor Ling. BA (first class) Biochemistry, Corpus Christi College, University of Oxford, UK.

The first of these to be identified was P-glycoprotein (P-gp), the product of the human MDR1 gene, localized to chromosome 7q P-gp is a member of the large ATP-binding cassette (ABC) family Cited by:   P-glycoprotein Structure and function. P-gp, a kDa membrane protein, was discovered in by Juliano and Ling as a phosphoglycoprotein expressed in Chinese hamster ovary cells R.

AllikmetsComplete characterization of the human ABC gene family. J Bioenerg Biomembr, 33 (6) (), pp. Google Scholar. (): Expression of the mdr (P-glycoprotein) gene in Chinese hamster digestive tracts. J Natl Cancer Inst – CAS Article Google Scholar.

Jane A. Endicott, Farida Sarangi, Victor Ling. Complete cDNA sequences encoding the Chinese hamster P-glycoprotein gene family. DNA Sequence2 (2), DOI: / biosurveillance studies for the detection of antiviral antibodies in wildlife, livestock and humans.

A variety of tools are used in the laboratory including animal cell expression and purification of viral membrane glycoproteins, cell-cell reporter gene fusion assays, monoclonal antibody development and viral reverse genetic systems.

The coronavirus spike protein is a multifunctional molecular machine that mediates coronavirus entry into host cells. It first binds to a receptor on the host cell surface through its S1 subunit and then fuses viral and host membranes through its S2 subunit.

Two domains in S1 from different coronaviruses recognize a variety of host receptors, leading to viral attachment. The spike protein. Keates RA, Sarangi F, Ling V. Structural and functional alterations in microtubule protein from Chinese hamster ovary cell mutants.

Proc Natl Acad Sci U S A. Sep; 78 (9)– [PMC free article] Kelley SL, Basu A, Teicher BA, Hacker MP, Hamer DH, Lazo JS. Overexpression of metallothionein confers resistance to anticancer drugs.

Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins.

P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation (CD) is an important protein of the cell membrane that pumps many foreign substances out of cells.

More formally, it is an ATP-dependent efflux pump with broad substrate. Ling V: A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim Biophys Acta (). Multidrug resistance in breast cancer: a meta-analysis of MDR1/gp expression and its possible functional significance.

Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA Katsuhiro Mori Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3‐6‐6 Asahi‐machi, Machida‐shi, Tokyo ‐, Japan; telephone: + 81‐42‐‐; fax: + 81‐42‐‐ To improve the clearance of these species, we developed a family of synthetic peptides that target HCPs and exhibit low binding to IgG product.

In this study, these peptides were conjugated onto chromatographic resins and evaluated in terms of HCP clearance and mAb yield, using an industrial mAb‐producing CHO harvest as model supernatant.

The ATP-binding cassette (ABC) transporters constitute a diverse gene family consisting of proteins found in all cellular organisms and participating in several different biological pathways [].Among these processes, the ABC transporters are mostly involved in extra and intracellular trans membrane ATP energy driven traffic of molecules such as lipids, amino acids, hormones and.

chinese hamster (V max) Evidence from studies on the functional exchangeability of the N- and C-terminal ABC domains of P-glycoprotein also suggested to the authors that residues in the helical domain might participate in signal transduction between the nucleotide-binding site and the transmembrane domains or, alternatively, might.

Purchase ABC Transporters: Biochemical, Cellular, and Molecular Aspects, Volume - 1st Edition. Print Book & E-Book. ISBNFor example, studies using molecular probes to P-glycoprotein messenger RNA and monoclonal antibodies to different epitopes of the molecule have shown that P-glycoprotein is expressed at high.

Objectives. Multidrug resistance (MDR) is the major reason for the failure of chemotherapy in colorectal cancer (CRC), and the primary determinant of MDR in CRC patients is active drug efflux owing to overexpression of P-glycoprotein (P-gp) in cancer tissues.

Despite research efforts to overcome P-gp-mediated drug efflux, the high toxicity of P-gp inhibitors has been a major obstacle for the.Many marine organisms inherently express the presence and function of a new defence mechanism, termed multixenobiotic resistance mechanism (MXRM) because of its similarity to multidrug resistance (MDR) found in tumor cell lines resistant to chemotherapeutic drugs.

However, previously no information was available on a possible induction of the activity of MXRM in organisms living at polluted sites. Both p53 and multidrug transporters play important roles in chemoresistance. A transcriptional dependence of the Mdr1 gene promoter by p53 was first established a decade ago, and despite intense study, the pMdr1 relationship still remains vague in general model proposes that wild-type p53 down regulates, while mutant p53 up regulates, the Mdr1 promoter.